A better understanding of organic- inorganic phosphorus transformations is needed to improve management of phosphorus fertilization of cultivated soils. This study was conducted to determine if the mineralization of organic phosphorus compounds in a soil could be induced enzymatically and observed using31Phosphorus Nuclear Magnetic Resonance Spectroscopy (31P NMR). Sampler, of an Osorno series (Typic Dystrandept) soil were sterilized using gamma radiation, then incubated with alkaline phosphatase, acid phosphatase, phosphodiesterase, and phytase. Soil extracts were prepared using 1.0MKOH, then treated with a cation exchange resin to remove interfering paramagnetics.31P NMR analysis of untreated and treated soil extracts showed a decrease in phytic acid and a corresponding increase in orthophosphate for the phytase-treated sample. Results indicate that soil organic P is mineralized enzymatically and that the NMR technique is useful for studying mineralization in soils having sufficiently high levels of organic P