The Use of Synthetic Peptides in the Formation of Biophysically and Biologically Active Pulmonary Surfactants
作者:
SUSAN REVAK,
T. MERRITT,
MIKKO HALLMAN,
GREGORY HELDT,
ROBERT LA POLLA,
KENWAY HOEY,
RICHARD HOUGHTEN,
CHARLES COCHRANE,
期刊:
Pediatric Research
(OVID Available online 1991)
卷期:
Volume 29,
issue 5
页码: 460-465
ISSN:0031-3998
年代: 1991
出版商: OVID
数据来源: OVID
摘要:
Synthetic pulmonary surfactants consisting of mixtures of phospholipids with synthetic peptides based on the amino acid sequence of human surfactant apoprotein SP-B were prepared. These surfactants were analyzed for their ability to lower surface tension on a pulsating bubble surfactometer and for their capacity to improve lung compliance and increase alveolar expansion in a fetal rabbit model of surfactant deficiency. The data demonstrate that several peptides, ranging from 17 to 45 residues in length, matching the carboxy-terminal sequence of the SP-B protein, when appropriately recombined with the phospholipids dipalmitoylphosphatidycholine and phosphatidylglycerol (3:1), are capable of producing a synthetic surfactant with biophysical and biologic activity approaching that of human surfactant derived from amniotic fluid.
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