Preparation of nematodes for scanning electron microscopy
作者:
C. D. Green,
A. R. Stone,
R. H. Turner,
S. A. Clark,
期刊:
Journal of Microscopy
(WILEY Available online 1975)
卷期:
Volume 103,
issue 1
页码: 89-99
ISSN:0022-2720
年代: 1975
DOI:10.1111/j.1365-2818.1975.tb04540.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SUMMARYNematodes from the orders Tylenchida and Rhabditida were fixed and processed in several different ways for examination with the scanning electron microscope (SEM). Four processes produced good preparations of fixed nematodes. Drying from acetone was the simplest of these techniques and most useful for regions of the tylenchid nematodes supported by skeletal tissue. Critical point drying, a more complicated procedure, gave good preparations but they required special care in processing. Nematodes infiltrated with glycerol and a conducting agent were the most life‐like but were difficult to examine. Specimens infiltrated with an epoxy resin looked natural and this was the most promising process trie
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