Determination of13CO2/12CO2Ratio by IRMS and NDIRS
作者:
E. Barth,
I. Tugtekin,
H. Weidenbach,
U. Wachter,
J. Vogt,
P. Radermacher,
G. Adler,
M. Georgieff,
期刊:
Isotopes in Environmental and Health Studies
(Taylor Available online 1998)
卷期:
Volume 34,
issue 1-2
页码: 209-213
ISSN:1025-6016
年代: 1998
DOI:10.1080/10256019708036348
出版商: Taylor & Francis Group
关键词: Breath tests;carbon 13 isotope ratio mass spectrometry;isotope selective nondispersive infrared spectrometry
数据来源: Taylor
摘要:
Breath tests using13C-labelled substrates require the measurement of13CO2/12CO2ratio in breath gas samples. Next to isotope ratio mass spectrometry (IRMS), which is very sensitive but also complex and expensive, alternatively isotope selective nondispersive infrared spectrometry (NDIRS) can be used to determine the13CO2/12CO2ratio in expired breath. In this study we compared NDIRS- with IRMS-results to investigate whether the less expensive and very simply to operate NDIRS works as reliable as IRMS. For this purpose we applicated 1-13C-Phenylalanine to patients with advanced liver cirrhosis and healthy volunteers and took duplicated breath samples for IRMS and NDIRS at selected time points. Our data show a good correlation between these two methods for a small number of samples as required for simple breath tests. Longer series, where repeated measurements are required on the NDIRS instrument lead to a decreasing correlation. This indicates the superiority of IRMS concerning13CO2-kinetics over longer time periods.
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