Laser Light Scattering Immunoassay for Malaria
作者:
Priyaranjan Bhakat,
Arati Roy,
KunalB. Roy,
Anita Saxena,
HimadriB. Bohidar,
期刊:
Journal of Immunoassay
(Taylor Available online 1999)
卷期:
Volume 20,
issue 3
页码: 103-114
ISSN:0197-1522
年代: 1999
DOI:10.1080/01971529909349346
出版商: Taylor & Francis Group
关键词: Laser immunoassay;Malaria;Contin
数据来源: Taylor
摘要:
Laser light scattering immunoassay (LIA) was proposed as a prospective diagnostic method for the detection of antibody (or antigen) by monitoring the agglutination of antigen (or antibody) coated carrier particles using dynamic light scattering (DLS) as probe, LIA is a very sensitive assay as it can detect microscopic immune complexes even when antibody (or antigen) level is low. A sizeable number of human sera collected from malaria endemic areas and hospitals have been analysed by ELISA using Pf parasite lysate or a RESA derived synthetic peptide as antigen parallel to LIA using Pf antigen coated polystyrene latex beads. Comparative analysis of data suggests LIA to be as good as ELISA and possibly better in terms of sensitivity and simplicity. LIA can be a simple and inexpensive immunoassay suitable for field use and mass application.
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