Pseudoepidermis, Constructed in vitro, for Use in Toxicological and Pharmacological Studies
作者:
Rosa Maria Scavarelli-Karantsavelos,
Shahanara Zaman Saroya,
Frizell L. Vaughan,
Isadore A. Bernstein,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1990)
卷期:
Volume 3,
issue 2
页码: 115-125
ISSN:1660-5527
年代: 1990
DOI:10.1159/000210858
出版商: S. Karger AG
关键词: Keratinocyte culture;Epidermis;Cutaneous toxicity;Skin irritants;Bis-(β-chloroethyl) sulfide;Cutaneous vesicant;Salicylic acid;Benzoic acid
数据来源: Karger
摘要:
The purpose of this study was to establish the validity of the stratified, cornified keratinocyte culture as a model for investigating cutaneous toxicities. This pseudoepidermis, grown on a nylon membrane at the air-liquid interface, responded to topical application of a known vesicant similarly to the response of the tissue in vivo. Alterations in the morphology of the in vitro model also resembled pathological changes seen in in vivo models after exposure to this agent. The effects of the skin irritants benzoate and salicylate on protein and DNA synthesis in the culture were also similar to those observed in vivo.
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