Localization of glucose-6-phosphate dehydrogenase in mouse and man by in situ hybridization: evidence for a single locus and transposition of homologous X-linked genes
作者:
P.A. Martin-DeLeon,
S.F. Wolf,
G. Persico,
D. Toniolo,
G. Martini,
B.R. Migeon,
期刊:
Cytogenetic and Genome Research
(Karger Available online 1985)
卷期:
Volume 39,
issue 2
页码: 87-92
ISSN:1424-8581
年代: 1985
DOI:10.1159/000132113
出版商: S. Karger AG
数据来源: Karger
摘要:
By hybridizing a tritiated human genomic probe (pGD3) to metaphase chromosomes in situ, we have localized the gene for glucose-6-phosphate dehydrogenase (G6PD) in both the human and mouse complement. The locus on the intact human X chromosome is close to the telomere on the long arm, confirming the assignment based on studies of an X/autosome translocation in human-mouse hybrids. Although the signal:background ratio was reduced for the heterologous hybridization of the human probe to mouse metaphases, 20% of the grains were on the X chromosome and 93% of these were in the A region, relatively close to the centromere. The location of G6PD in mouse and man reflects intrachromosomal transposition of these homologous X loci. Genomic DNAs from mouse and man and from hybrids with human X/autosome translocations were digested with several restriction enzymes including EcoRl, PstI, and HpaII, and Southern blots were probed with 32P-pGD3. The results of the analysis also confirm the human G6PD assignment and are consistent with a single copy of the locus in the haploid genome of both species.
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