A simple quantitative HPLC method for determination of aflatoxins in cereals and animal feedstuffs using gel permeation chromatography clean‐up
作者:
MichaelT. Hetmanski,
KeithA. Scudamore,
期刊:
Food Additives & Contaminants
(Taylor Available online 1989)
卷期:
Volume 6,
issue 1
页码: 35-48
ISSN:0265-203X
年代: 1989
DOI:10.1080/02652038909373737
出版商: Taylor & Francis Group
关键词: Aflatoxins;cereals;animal feedstuffs;gel permeation chromatography;HPLC
数据来源: Taylor
摘要:
A simple and sensitive procedure is described for the analysis of aflatoxins B1B2, G1and G2in cereal and animal feedstuff samples. Aflatoxins are extracted with dichloromethane:water (10:1). Gel permeation chromatography (GPC) using a column packed with Bio‐beads S‐X3 and dichloromethane:hexane (3:1) as the eluent is used for clean‐up of extracts prior to separation and quantification of aflatoxins by HPLC. The eluent fraction containing the aflatoxins is concentrated by evaporation under reduced pressure and the aflatoxins separated by reverse phase HPLC on an ODS column. Quantitative results have been obtained at levels down to 1μg/kg, and average recoveries from samples of wheat, maize and compound feed spiked at levels of 10 and 40μg/kg were greater than 80%, 70% and 65% respectively.
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