Identification of Anti-Idiotypic Antibodies to Anti-Phosphotyrosine Antibodies in Human Sera
作者:
StefanescuMaria,
MatacheCristiana,
OnuAdrian,
CristescuCarol,
CremerLidia,
SzegliGeza,
期刊:
Autoimmunity
(Taylor Available online 1993)
卷期:
Volume 15,
issue 3
页码: 181-186
ISSN:0891-6934
年代: 1993
DOI:10.3109/08916939309019925
出版商: Taylor&Francis
关键词: SLE;phosphotyrosine;anti-idiotype
数据来源: Taylor
摘要:
We have recently identified in SLE sera antibodies against phosphotyrosine. They were also detected in normal sera and gammaglobulin preparations, suggesting that they belong to natural autoantibodies.In this paper, the occurrence of anti-idiotypic antibodies against anti-phosphotyrosine antibodies, in the above mentioned samples, is investigated. In order to identify these anti-idiotypic antibodies ELISA, immunoprecipitation and immunoblotting are performed.Our data demonstrate the presence of anti-idiotypic antibodies specific to anti-phosphotyrosine antibodies in SLE sera as well as in normal sera, suggesting that these anti-idiotypic antibodies are also auto-anti-idiotypic antibodies. The densitometry of immunoblots reveals significantly higher levels of anti-idiotypic antibodies in SLE sera. Based on the competition inhibition studies we conclude that some of these anti-idiotypic antibodies belong to beta/gamma type.
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