Lysozyme loading and release from hydrogels carrying pendant phosphate groups
作者:
Katsuhiko Nakamae,
Takeshi Nizuka,
Takashi Miyata,
Manabu Furukawa,
Takashi Nishino,
Koichi Kato,
Tadaaki Inoue,
Allan S. Hoffman,
Yoshio Kanzaki,
期刊:
Journal of Biomaterials Science, Polymer Edition
(Taylor Available online 1998)
卷期:
Volume 9,
issue 1
页码: 43-53
ISSN:0920-5063
年代: 1998
DOI:10.1163/156856297X00254
出版商: Taylor & Francis Group
关键词: Protein loading;hydrogel;polyelectrolyte;Phosmer M;lysozyme;polyion complex;protein drug delivery
数据来源: Taylor
摘要:
—To develop a polymeric matrix for efficiently loading cationic biomolecules, polyelectrolyte hydrogels carrying pendant phosphate groups were synthesized by copolymerizing 2-methacryloyloxyethyl dihydrogen phosphate with N-isopropylacrylamide and N,N'-methylene-bis-acrylamide. The phosphate-carrying monomer yielded anionic hydrogels, which formed ionic complexes with the cationic protein, lysozyme. It was shown that the amount of complexed lysozyme reached 2.1 gg-1dry gel, corresponding to 1.3 x 10-3mol phosphate group per gram lysozyme, when 40 mol% of phosphate-carrying monomer was incorporated in a hydrogel. When the hydrogel complexed with lysozyme was placed in deionized water and various KCl solutions, of varying concentrations of up to 0.5 M KCl, no lysozyme was released in deionized water, while increasing amounts of lysozyme were released as the KCl concentration increased. This confirmed that lysozyme was loaded in the hydrogel through electrostatic interactions. It was further found that the complexed lysozyme retained its enzymatic activity after being released from the hydrogel. These results suggest the use of this system for the controlled release of cationic protein drugs.
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