New researches on the culture « in vitro » of Aucuba japonica Thunb, – Caulinar fragments grownin vitroin nutrient media solidified with agar are compared with fragments grown on a filter paper support on unshaken liquid media. Tissue cultures ofAucubaare established putting down caulinar fragments just taken from the plant with isolated callus; subcultures are obtained more easily in July. We have observed that during the months of March or April, caulinar fragments, taken from the plant at various months of the year, of different ages and cultivated on various media, unexpectedly produce many roots.