HIV-1-specific mucosal IgA in a cohort of HIV-1-resistant Kenyan sex workers
作者:
Rupert Kaul,
Daria Trabattoni,
Job Bwayo,
Donatella Arienti,
Arianna Zagliani,
Francis Mwangi,
Cecilia Kariuki,
Elizabeth Ngugi,
Kelly MacDonald,
T. Ball,
Mario Clerici,
Francis Plummer,
期刊:
AIDS
(OVID Available online 1999)
卷期:
Volume 13,
issue 1
页码: 23-29
ISSN:0269-9370
年代: 1999
出版商: OVID
关键词: Africa;HIV-1 resistance;commercial sex workers;genital tract;humoral immunity;cellular immunity;sexual behaviour
数据来源: OVID
摘要:
Objectives:Most HIV-1 transmission is sexual; therefore, immune responses in the genital mucosa may be important in mediating protection against HIV infection. This study examined HIV-1-specific mucosal IgA in a cohort of HIV-1-resistant Kenyan female sex workers.Methods:HIV-1-specific immune responses were compared in HIV-1-resistant and HIV-1-infected sex workers, and in lower risk uninfected women. Cervical and vaginal samples from each group were tested for HIV-1-specific IgA and IgG by enzyme immunoassay. Systemic T-helper lymphocyte cell responses to HIV-1 envelope peptide epitopes were assayed using an interleukin 2 bioassay. HIV-1 risk-taking behaviours were assessed using standardized questionnaires.Results:HIV-1-specific IgA was present in the genital tract of 16 out of 21 (76%) HIV-1-resistant sex workers, five out of 19 (26%) infected women, and three out of 28 (11%) lower risk women (P< 0.0001). Among lower risk women, the presence of HIV-1-specific IgA was associated with HIV-1 risk-taking behaviour. Systemic T-helper lymphocyte responses to HIV-1 envelope peptides were present in 11 out of 20 (55%) HIV-1-resistant women, four out of 18 (22%) infected women, and one out of 25 (4%) lower risk women (P< 0.001). T-helper lymphocyte responses did not correlate with the presence or titre of virus-specific mucosal IgA in any study group.Conclusions:HIV-1-specific IgA is present in the genital tract of most HIV-1-resistant Kenyan sex workers, and of a minority of lower risk uninfected women, where it is associated with risk-taking behaviour. These data suggest a role for mucosal HIV-1-specific IgA responses in HIV-1 resistance, independent of host cellular responses.
返 回