SummaryA new method for allatectomy of queen honeybees is described. This technique makes it possible to carry out the allatectomy in the air instead of under physiological solution. After allatectomy, three young queens lived for 22, 35 and nearly 50 days, respectively, and during this time they laid eggs; the two longer-lived queens showed a fairly normal rate of brood production up to about 10 days before death, when the rate decreased. In the period following allatectomy, the workers continued to form a retinue around each queen, and no queen cells were produced in the colonies. This would seem to indicate that production of pheromone by the queens proceeded adequately for the colonies used.