Regional assignment of the human 4-hydroxyphenylpyruvate dioxygenase gene (HPD) to 12q24→qter by fluorescence in situ hybridization
作者:
G. Stenman,
E. Röijer,
U. Rüetschi,
A. Dellsén,
L. Rymo,
S. Lindstedt,
期刊:
Cytogenetic and Genome Research
(Karger Available online 1995)
卷期:
Volume 71,
issue 4
页码: 374-376
ISSN:1424-8581
年代: 1995
DOI:10.1159/000134142
出版商: S. Karger AG
数据来源: Karger
摘要:
Using a panel of human-rodent somatic cell hybrids, we have previously mapped the gene (HPD, previously called PPD) encoding 4-hydroxyphenylpyruvate dioxygenase to the distal half of the long arm of human chromosome 12, region q14→qter. To obtain a genomic probe useful for fluorescence in situ hybridization (FISH) analysis we screened a human leukocyte genomic library and isolated a 13.4-kb phage clone, which by restriction fragment and sequence analyses was shown to contain exons 1–10 of HPD and approximately 2-kb upstream sequences. We now report the subregional localization of HPD to 12q24→qter based on two color FISH analysis employing this
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