AbstractSegments of nicotinic acetylcholine receptor α sub‐unit genes have been isolated from a panel of insect species by polymerase chain reaction, using degenerate oligonucleotide primers designed to recognize conserved regions of theDrosophila melanogasterALS and SAO genes. The amplified segments encode elements of typical a‐subunits anticipated to play roles in ligand binding and ion channel formation. Each is also clearly either ALS or SAD‐like. The predicted protein sequences display extremely high levels of conservation (over 85% for each subtype) even though derived from very distantly related insect s