PREPARATIVE CHROMATOGRAPHIC SEPARATION OF TAXOL FROM YEW TREES
作者:
YoungKwang Park,
SungTaik Chung,
KyungHo Row,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 18
页码: 2755-2761
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100102056
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Taxol shows promise for the treatment of a variety of human cancers, including leukemia and certain ovarian, breast, and lung tumors. In this work, the separation of taxol from Korean Yew tree was studied with solvent extraction and liquid chromatography. The content of taxol in the tree was analyzed to be 0.064 mg/g of dry powder. Methanol was used to extract taxol and the resulting extract was concentrated by the partition of water and methylene chloride. In the pretreatment step with open tubular chromatography, the first mobile phase was methylene chloride/acetone, 97/3 vol. % and the second mobile phase methylene chloride/acetone, 75/25 vol. %. The sample containing taxol was further purified by varying the composition of solvent, flow rate, and injection amount in analytical chromatography.
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