Fiber Optic Fluorescence Scanning in Thin-Layer Chromatography
作者:
A.Navas Diaz,
F.Garcia Sanchez,
期刊:
Instrumentation Science & Technology
(Taylor Available online 1994)
卷期:
Volume 22,
issue 3
页码: 273-281
ISSN:1073-9149
年代: 1994
DOI:10.1080/10739149408000455
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The effectiveness of fluorometric scanning using a fiber optic to analyze substances adsorbed onto solid surfaces is described. The relative efficiencies of signal capture by different widths of monochromator slits were studied. The signal and signal-to-noise ratios were compared using monochromators or long-pass filters. Accuracy and precision of the measurements were compared with and without substraction of the background signal; the detection limits for fluorescein and several phenothiazine compounds were compared under different experimental conditions to explore the range of effectiveness of the system.
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