Radioimmunoassay of Gentamicin in Microliter and Nanoliter Samples of Biological Fluids
作者:
A. Meulemans,
C. Manuel,
Tran Ba Huy,
期刊:
Chemotherapy
(Karger Available online 1981)
卷期:
Volume 27,
issue 1
页码: 29-33
ISSN:0009-3157
年代: 1981
DOI:10.1159/000237951
出版商: S. Karger AG
关键词: Radioimmunoassay;Gentamicin;Inner ear fluids;Rats
数据来源: Karger
摘要:
A gentamicin radioimmunoassay is described for microliter and nanoliter samples. The assay was performed in the inner ears’ fluids (perilymph and endolymph) of rats. Endolymph and perilymph samples were collected under oil with a glass capillary micropipette. The absolute threshold of sensitivity was 20 · 10––12 g. Considering the maximum sampling volume, 15–400 · 10––9 and 10 · 16––6 l for endolymph and perilymph respectively, the limit of detection is 4.19 · 10––4μM · ml––1 in endolymph, 1.26 · 10––4μM · ml––1 in perilymph. Clinical a
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