Phagocytosis of Protein Coated Colloidal-Gold-Agarose-Gelatin Microbeads by Cultured Uterine Glandular Epithelial and Stromal Cells
作者:
GaoKuixiong,
SmithScott E.,
GodkinJames D.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 1
页码: 1-6
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110540
出版商: Taylor&Francis
关键词: epithelial cells;colloidal gold;endo-cytosis;immunocytochemistry;microbeads
数据来源: Taylor
摘要:
A procedure for fixing and immunostaining whole cells from primary cultures of ovine and bovine uterine gland fragments was used to identify keratin in intermediate filaments of epithelial cells to distinguish them from stromal cells. Colloidal gold encapsulated aga-rose-gelatin microbeads were coated with different proteins and used to investigate uptake by epithelial and stromal cells in culture. Micro-beads were taken up by stromal cells and by epithelial cells on the outskirts of colonies. These cells formed ridges where they contacted and grew above stromal cells. Electron microscopy demonstrated that the microbeads had been internalized and appeared to be nontoxic. Individual cells could harbor more than 90 microbeads within their cytoplasm for at least seven to ten days with no apparent harm. Some cells with microbeads were seen to divide.
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