Short-lived radioisotopes separated from radionuclide generators are widely used. In the paper possibilities of labelling with the daughter nuclides of the commercially available Mo/Tc-, Sn/In- and Te/I-generators and of the self-made Ba/La-generator in industrial tracer experiments are presented. The transfer of the daughter nuclides from the generator eluates into organic phases and the labelling of oil-phases and solid particles are investigated. The developed simple, quick and efficient methods are suitable for routine application under industrial conditions. Some examples of industrial applications of the generator nuclides are given, too.