Separation and Determination of D-Gluconic Acid Produced During Fermentation by Capillary Zone Electrophoresis
作者:
ZiPing Zhang,
ZhiDe Hu*,
GengLiang Yang,
期刊:
Analytical Letters
(Taylor Available online 1996)
卷期:
Volume 29,
issue 11
页码: 2025-2037
ISSN:0003-2719
年代: 1996
DOI:10.1080/00032719608000809
出版商: Taylor & Francis Group
关键词: D-gluconic acid;capillary zone electrophoresis;fermentation
数据来源: Taylor
摘要:
A novel method was developed for separation and determination of D-gluconic acid produced during fermentation by capillary zone electrophoresis (CZE) with direct UV detection at 214 nm, using selected carrier electrolyte composed of 6 mM potassium biphthalate, 50 mM disodium hydrogen phosphate and 15% (v/v) acetonitrile. The effects of concentration of phthalate, phosphate and organic modifier (acetonitrile), as well as temperature for the separation were investigated. The method is simple, inexpensive and will make it very useful in the gluconic acid industry.
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