Glycosaminoglycans of Organ-Cultured Rabbit Trabecular Meshwork
作者:
Keizo Nishiyama,
期刊:
Ophthalmologica
(Karger Available online 1992)
卷期:
Volume 204,
issue 1
页码: 35-43
ISSN:0030-3755
年代: 1992
DOI:10.1159/000310264
出版商: S. Karger AG
关键词: Glycosaminoglycans;Trabecular meshwork;Organ culture;Electrophoresis;Enzymatic degradation;Radiolabel precursor;Rabbit eye
数据来源: Karger
摘要:
The anterior segments of rabbit eyes were dissected into two types of corneoscleral explants: one a large ring-shaped block of tissue from one eye and the other consisting of small blocks of tissue from the other eye which was dissected into 8 parts. Explants were cultured for up to 2 weeks. The glycosaminoglycan (GAG) profiles of the organ-cultured rabbit trabecular meshwork were then investigated and compared with respect to explant size. The results revealed: (1) similar trabecular GAG profiles in both sizes of explants, (2) trabecular GAG profiles obtained within the culture period came to resemble those of noncultured specimens and (3) incorporation of the radiolabels continued for up to 5 days, with 2 days sufficient for GAG analysis.
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