Peroxidase–ferrocene modified carbon paste electrode as an amperometric sensor for the hydrogen peroxide assay
作者:
Pilar Domínguez Sánchez,
Arturo José Miranda Ordieres,
Agustín Costa García,
Paulino Tuñón Blanco,
期刊:
Electroanalysis
(WILEY Available online 1991)
卷期:
Volume 3,
issue 4‐5
页码: 281-285
ISSN:1040-0397
年代: 1991
DOI:10.1002/elan.1140030407
出版商: VCH Verlagsgesellschaft mbH
数据来源: WILEY
摘要:
AbstractA new enzymatic amperometric sensor for hydrogen peroxide based on a modified carbon paste electrode is described. Horseradish peroxidase and ferrocene mediator were immobilized by dissolution or dispersion in a graphite–paraffin oil paste. To prevent losses of immobilized chemicals under operational conditions, the electrode surface was coated with a Nafion membrane. The device exhibits a relatively fast response time (42 s for 95% of the steady‐state response) and yields currents linearly related to the hydrogen peroxide concentration over the 10−7to 10−5mol/L range in both static and flow injection operations. The slope of the calibration plot remained constant for more than 20 days. Flow injection assays of hydrogen peroxide at a sampling rate of about 70 injections per hour, with a relative standard deviation of 1.4%, are possible. An easy and low cost preparation procedure is de
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