Gene mapping by microdissection and enzymatic amplification: Heterogeneity in leukaemia associated breakpoints on chromosome II
作者:
Finbarr E. Cotter,
Debra Lillington,
Garret Hampton,
Peter Riddle,
Soma Nasipuri,
Barbara Gibbons,
Bryan D. Young,
期刊:
Genes, Chromosomes and Cancer
(WILEY Available online 1991)
卷期:
Volume 3,
issue 1
页码: 8-15
ISSN:1045-2257
年代: 1991
DOI:10.1002/gcc.2870030103
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA new strategy for mapping chromosome translocation breakpoints in relation to known genes has been developed. This approach is based on the amplification by the polymerase chain reaction (PCR) of specific target sequences from small numbers of microdissected chromosome fragments. This method has been applied to leukaemia‐associated translocations affecting the q23 region of chromosome I I. In two independent leukaemias, the t(6; 11) translocation was distinguished from the t(9; 11) and t(4; 11) translocations by demonstrating that the former breakpoint on chromosome 11 lay proximal to the CD3D gene while the latter breakpoints lay distal to CD3D. All three translocation breakpoints were found to lie proximal to ETSl and THYl. The data suggest that although these leukaemia‐associated breakpoints on chromosome 11 are cytogenetically identical they may involve disruption of different genes. This approach offers a rapid alternative to mapping by hybridisation of probes either in situ to chromosomes or to somatic cell hybrids containing the appropriate derivative chromoso
点击下载:
PDF
(1588KB)
返 回