Effect of hylan on cartilage and chondrocyte cultures
作者:
Nancy E. Larsen,
Kathleen M. Lombard,
Edward G. Parent,
Endre A. Balazs,
期刊:
Journal of Orthopaedic Research
(WILEY Available online 1992)
卷期:
Volume 10,
issue 1
页码: 23-32
ISSN:0736-0266
年代: 1992
DOI:10.1002/jor.1100100104
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Hylan;Hyaluronan;Articular cartilage;Chondrocytes;Proteoglycan;Polymorphonuclear leukocytes;Oxygen‐derived free radicals
数据来源: WILEY
摘要:
AbstractThe protective role of hylan, a hyaluronan [hyaluronic acid (HA)] derivative, was studied in explanted bovine cartilage and isolated chondrocytes. Cartilage and chondrocytes were exposed to degradative enzymes (lysate from activated polymorphonuclear leukocytes), oxygen‐derived free radicals (ODFR), conditioned media from mononuclear cells (MCCM), and interleukin‐1 (IL‐1), in the presence and absence of hylan. The effect of HA was also studied. In cartilage explants susceptibility to pertubation was evaluated in terms of35S release and proteoglycan depletion and was compared to control cultures; high viscosity hylan was found to reduce35S release in cartilage explants caused by degradative enzymes, ODFR, MCCM, and IL‐1. The hylan effect was reversible and viscosity‐dependent. In chondrocyte cultures, high viscosity hylan was effective in reducing cell injury caused by degradative enzymes and ODFR. The data suggest that the glycosaminoglycan hylan, as well as native HA, may mediate exposure to and/or response to stimuli associated with initiation of degenerative processes in cartilag
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