Rapid Amperometric Determination of Magnesium(II) Ions
作者:
D. Compagnone,
G. G. Guilbault,
期刊:
Analytical Letters
(Taylor Available online 1996)
卷期:
Volume 29,
issue 4
页码: 505-514
ISSN:0003-2719
年代: 1996
DOI:10.1080/00032719608000417
出版商: Taylor & Francis Group
关键词: magnesium(II);glucose electrode;hexokinase;amperometry
数据来源: Taylor
摘要:
An amperometric procedure for the measurement of magnesium(II) has been developed using the enzyme hexokinase in solution and glucose oxidase immobilized onto a preactivated polymeric support. The reaction of hexokinase was monitored following the decrease in current due to the glucose consumption by the enzyme in the presence of the ATP-Mg2+complex. The reaction rate was dependent on the concentration of magnesium(II) in solution. Concentrations of hexokinase and ATP were optimised. Measuring the current change in the 1-3 min interval after the start of the reaction magnesium(II) can be determined in the 4 × 10−5to 10−3M range. Other divalent cations tested showed no interference. The magnesium(II) content of 5 pharmaceutical products was measured with the amperometric and compared to a spectrophotometric procedure. The results correlated well.
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