Effects of Recombinant Interleukin‐2 on Resting Human T Lymphocytes
作者:
Scott Hammer,
Jacqueline Gillis,
期刊:
Journal of Biological Response Modifiers
(OVID Available online 1986)
卷期:
Volume 5,
issue 1
页码: 36-44
ISSN:0732-6580
年代: 1986
出版商: OVID
关键词: Human T cells;Interleukin-2
数据来源: OVID
摘要:
The effects of recombinant interleukin-2 on resting T lymphocytes were examined in vitro. T cell enriched populations were isolated from the peripheral venous blood of three normal individuals by Ficoll-Hypaque gradient separation and standard sheep erythrocyte rosetting techniques. Interleukin-2 at concentrations of 100 and 1,000 U/ml stimulated tritiated thymidine incorporation, Tac antigen expression, interferon production, and blast transformation in T cell cultures. These changes were observed in the absence of cell proliferation. The effects of interleukin-2 at a concentration of 100 U/ml were substantially blocked by the addition of a 1:1,000 dilution of anti-Tac antibody to the culture medium indicating that the effects observed were mediated by the Tac receptor. These results indicate that recombinant interleukin-2 can have demonstrable functional and morphologic effects on unstimulated human T cells.
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