AbstractYoung rat pups are dependent on the dam for their survival, thus isolation of the neonatal rat pup from the dam presents the young organism with a variety of stressors. The question examined in this study concerns the ability of the young rat pup to modify its response to isolation following repeated exposure to that isolation as well as the role played by endogenous opiates in this process. Following repeated isolations, pups were seen to decrease vocalization rates. Altering the context in an attempt to dishabituate animals failed to reverse the decreased vocalization rate. However, opiate receptor blockade attenuated this decrease when administered subsequent to the first isolation period but not prior to the last isolation period. These results suggest that the development of this attenuated response to isolation stress is opiate‐mediated but that once established, its expression is not dependent on endogenous opiate release.© 1994 John Wiley&Sons, I