Light and Electron Microscopic Correlation of Human Amniotic Fluid Cells in Late Pregnancy
作者:
STAFFAN BERGSTROM,
OLOF TYDEN,
BO NILSSON,
期刊:
Obstetrics & Gynecology
(OVID Available online 1982)
卷期:
Volume 59,
issue 2
页码: 176-181
ISSN:0029-7844
年代: 1982
出版商: OVID
数据来源: OVID
摘要:
Cytologic examination of oxazone-stained (nile blue) samples of amniotic fluid has become increasingly accepted as a reliable, inexpensive, and simple method of assessing fetal maturity. The present study focused on the ultrastructure of amniotic fluid cells in late human pregnancy. By means of light microscopy and scanning electron microscopy of the same cell in a series of cells, it was possible to observe the ultrastructural counterpart to the cell pattern indicative of fetal maturity. The type of cell indicating immaturity had a cytoarchitecture different from that of cells indicating maturity. The latter had an amorphous surface and coarse cytoplasmic strands; their oxazone-stainable, orange fat droplets were not found at electron microscopy. It is concluded that cells indicating maturity and those indicating immaturity differ in ultrastructure.
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