Melatonin Determination in Human Urine by Liquid Chromatography Using Flurometric Detection
作者:
M. Hariharan,
J.S. Naickar,
A.S. Hilibrand,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1996)
卷期:
Volume 19,
issue 11
页码: 1745-1756
ISSN:1082-6076
年代: 1996
DOI:10.1080/10826079608014002
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A new, simple, very sensitive, highly reproducible, isocratic, and reverse phase liquid chromatographic method has been developed for determining urine melatonin concentrations using a fluorometric detector. 6-methoxymelatonin is used as an internal standard. A C18column (150 × 0.46 cm) and a “Waters” fluorometric detector (Model 474 with a 16 uL flow cell are the main components of the liquid chromatographic system. The excitation and emission wavelengths are 285 and 335 nm respectively. Melatonin is extracted from urine at pH 7 using chloroform. The method is sensitive to 0.5 pg. The mean (n=6) inter-assay precision (CV) is 4.3% and intra-assay precision is 5.8%. The mean absolute recovery of the method is about 90%.
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