The Expression of PDGFα- andβ-Receptors in Subpopulations of PDGF-Producing Cells Implicates Autocrine Stimulatory Loops in the Control of Proliferation in Cytotrophoblasts that Have Invaded the Maternal Endometrium
作者:
HolmgrenLars,
ClaessonLena,
HenrikCarl,
OhlssonRolf,
期刊:
Growth Factors
(Taylor Available online 1992)
卷期:
Volume 6,
issue 3
页码: 219-231
ISSN:0897-7194
年代: 1992
DOI:10.3109/08977199209026929
出版商: Taylor&Francis
关键词: PDGF;platelet-derived growth factor;in situ hybridization;cytotrophoblasts;placenta
数据来源: Taylor
摘要:
AbstractIn order to explain the high proliferative potential of human placental cytotrophoblasts, we have addressed the potential involvement of platelet-derived growth factor (PDGF) ligand and receptors. Although PDGF is usually described as a mitogen for cells of mesenchymal origin, we show in this report that extra-villous term placental cytotrophoblasts express the PDGFα- andβ-receptor genes, both in vivo and in vitro. In addition, cytotrophoblasts produce significant amounts of PDGF-B protein. By immunohistochemical analysis of receptor expression, we found that the PDGF a-receptors could be detected at the cell surface, while the PDGFβ-receptors were only detected inrracellularly. In addition, double immunostaining analysis showed that the PDGFα- andβ-receptor molecules are expressed in different subpopulations of cytotrophoblasts. The addition of PDGF-AA and PDGF-BB homodimers to cytotrophoblast primary cultures induced a significant increase in DNA synthesis. We conclude, therefore, that PDGF is a growth factor for placental cytotrophoblasts and suggest that the growth of cytotrophoblasts can partly be explained by a PDGF autostimulatory loop, limited by the number of receptor-positive cytotrophoblasts.
点击下载:
PDF (1622KB)
返 回