High-Resolution Immunostaining of Malignancies by Computerized Robotic Capillary Action
作者:
MasonDavid L.,
PedrazaMiguel A.,
BoblettJohn P.,
DosluFeridun A.,
MarshRichard A.,
期刊:
Journal of Histotechnology
(Taylor Available online 1990)
卷期:
Volume 13,
issue 4
页码: 249-254
ISSN:0147-8885
年代: 1990
DOI:10.1179/his.1990.13.4.249
出版商: Taylor&Francis
关键词: computer program;high-resolution immunomicroscopy;malignancy;robotic capillary action
数据来源: Taylor
摘要:
AbstractHigh-resolution immunolight microscopy was carried out on a series of neoplasms embedded in Spurr plastic by means of computerized robotic capillary action. The techniques and computer program are delineated. The results demonstrate the highly resolved immunostaining with low background attained in plastic sections. Tissues were dewaxed, reembedded in Spurr, and reacted on the autostainer with no qualitative loss of immunoreactivity. Advantages of employing the autostainer include quality control (both positive controls and unknowns can be immunoreacted in tandem), ability to carry out immunoreactions on different cases at the same time, use of far less quantities of antibody and reagents than for manual procedures, and better use of technologist time (other laboratory procedures can be done while the sections are being robotically stained). (The J Histotechtzol13:249, 1990)
点击下载:
PDF (1786KB)
返 回