首页   按字顺浏览 期刊浏览 卷期浏览 Occurrence of whitefly‐transmitted geminiviruses in crops in Burkina Faso, and their se...
Occurrence of whitefly‐transmitted geminiviruses in crops in Burkina Faso, and their serological detection and differentiation

 

作者: G KONATÉ,   N BARRO,   D FARGETTE,   M M SWANSON,   B D HARRISON,  

 

期刊: Annals of Applied Biology  (WILEY Available online 1995)
卷期: Volume 126, issue 1  

页码: 121-129

 

ISSN:0003-4746

 

年代: 1995

 

DOI:10.1111/j.1744-7348.1995.tb05008.x

 

出版商: Blackwell Publishing Ltd

 

关键词: Bemisia tabaci;cassava;geminivirus;monoclonal antibody;okra;tobacco;tomato

 

数据来源: WILEY

 

摘要:

SummaryWhitefly‐transmitted geminiviruses were found to be associated with four diseases of crop plants in Burkina Faso: cassava mosaic, okra leaf curl, tobacco leaf curl and tomato yellow leaf curl. Tomato yellow leaf curl is an economically serious disease, reaching a high incidence in March, following a peak population of the vector whitefly,Bemisia tabaci, in December. Okra leaf curl is also a problem in the small area of okra grown in the dry season but is not important in the main period of okra production in the rainy season. The geminiviruses causing these four diseases, African cassava mosaic (ACMV), okra leaf curl (OLCV), tobacco leaf curl (TobLCV) and tomato yellow leaf curl (TYLCV) viruses, were each detected in field‐collected samples by triple antibody sand‐wich‐ELISA with cross‐reacting monoclonal antibodies (MAbs) to ACMV. Epitope profiles obtained by testing each virus isolate with panels of MAbs to ACMV, OLCV and Indian cassava mosaic virus enabled four viruses to be distinguished. ACMV and OLCV had similar but distinguishable profiles. The epitope profile of TobLCV was the same as that of one form of TYLCV (which may be the same virus) and was close to the profile of TYLCV from Sardinia. The other form of TYLCV reacted with several additional MAbs and had an epitope profile close to that of TYLCV from Senegal. Only minor variations within each of these four types of epitope profile were found among geminivirus isolates from Burkina Faso.Sida acutais a wild hos

 

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