A simple and rapid screening method for the determination of protein and tryptophan in kernel halves and small samples of barley meal
作者:
Hannu Ahokas,
期刊:
Journal of the Science of Food and Agriculture
(WILEY Available online 1978)
卷期:
Volume 29,
issue 1
页码: 47-52
ISSN:0022-5142
年代: 1978
DOI:10.1002/jsfa.2740290108
出版商: John Wiley&Sons, Ltd
数据来源: WILEY
摘要:
AbstractThe protein of distal halves of barley kernels or small meal samples was extracted with ethanolic sodium hydroxide solution. After dilution, the protein content was estimated with a spectrophotometer by measurements at 215 and 225 nm. The tryptophan content was estimated from the same extract by a spectrophotofluorimeter. The methods showed high correlations with those used for control. The presented methods can be used in screening kernels in segregating ears or selecting single plants.
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