The silver staining procedure of sodium dodecyl sulfate‐gels may be accelerated by shortening fixation time
作者:
Svend Kirkeby,
Dennis Moe,
Thorkild C. Bòg‐Hansen,
期刊:
ELECTROPHORESIS
(WILEY Available online 1993)
卷期:
Volume 14,
issue 1
页码: 51-55
ISSN:0173-0835
年代: 1993
DOI:10.1002/elps.1150140109
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractIn most silver staining methods the first step in the staining of proteins separated after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis is a rather protracted fixation of the gels. Optimum fixation should be short, cause no background staining and effectively immobilize the proteins in the gel without masking the proteins for reaction with the staining solution. Further, the concentration of the fixing compounds should be as low as possible due to the potentional toxicity of fixatives. Fixation for only 5 min with mixtures of very low concentrations of formaldehyde and glutaraldehyde in ethanol, or a solution of formaldehyde or glutaraldehyde in picric acid and ethanol, fulfill these demands, provided that the gels were prefixed in ethanol‐acetic acid for 10 min. As a consequence of these results a fast and sensitive silver staining procedure is propo
点击下载:
PDF
(578KB)
返 回