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15N NMR of glycine metabolism in liver, muscle and kidney of rats using a new pulsed overhauser technique (POE)

 

作者: W. Gründer,   P. Krumbiegel,   K. Gersonde,  

 

期刊: Isotopenpraxis Isotopes in Environmental and Health Studies  （Taylor Available online 1992）
卷期: Volume 28, issue 1  

页码: 81-84

 

ISSN:0021-1915

 

年代: 1992

 

DOI:10.1080/00211919208050831

 

出版商: Taylor & Francis Group

 

关键词: Diagnostic techniques;glycine;metabolism;nitrogen 15;nuclear magnetic resonance spectroscopy;Overhauser techniques;rats

 

数据来源: Taylor

 

摘要:

Following intravenous injections of [15N]glycine (97 at.%15N, 400 mg in 1.5 ml isotonic saline) to Lewis rats several organs were excised. The time courses of the15N NMR spectral (40.55 MHz) were measured at 37°C after cold (4°C) or warm (37°C) storage. The application of the Overhauser POE technique yielded signal enhancement by a factor of -2.7 and -1.3 in sceletal and heart muscle, respectively, at 37°C, but no enhancement in liver and kidney.

 

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