Map of Sequential B Cell Epitopes of the HIV-1 Transmembrane Protein Using Human Antibodies as Probe
作者:
Jaap Goudsmit,
Rob H. Meloen,
Robert Brasseur,
期刊:
Intervirology
(Karger Available online 1990)
卷期:
Volume 31,
issue 6
页码: 327-338
ISSN:0300-5526
年代: 1990
DOI:10.1159/000150169
出版商: S. Karger AG
关键词: HIV-1;gp41;B cells;Antibodies;Epitopes;Pepscan
数据来源: Karger
摘要:
Antibodies of individuals infected with the human immunodeficiency virus type 1 (HIV-1) were used to probe the antigenicity of the HIV-1 transmembrane protein of 41 kD (gp41) by antibody-reactive peptide scanning (Pepscan). Eleven distinct sequential antibody-binding sites were defined by testing reactivity to 339 overlapping nonapeptides spanning the complete gp41 amino acid sequence. Such analysis only maps continuous antibody-binding sites of nine amino acids in length and does not identify putative discontinuous or assembled epitopes. Three B cell epitopes (aa 609–622; aa 655–699; aa 664–681) at the amino-terminal border of the putative transmembrane anchor and two (aa 732–748; aa 744–762) at the carboxyl-terminal border of this domain were the most antigenic. One antibody-binding domain (aa 834–852) with four amino acids homologous to the beta-1 domain of HLA class II beta-chain was recognized by the serum in 1 of 4 AIDS patients tested and not by any of the eight sera from symptom-free individuals. Although functional domains of gp41 involved in virus replication, cytopathicity and possibly immunosuppression were shown to bind antibodies of HIV-1-infected individuals, no relationship between antibody recognition patterns and disease progression w
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