首页   按字顺浏览 期刊浏览 卷期浏览 Somatic embryo development in carrot is associated with an increase in levels of S‐aden...
Somatic embryo development in carrot is associated with an increase in levels of S‐adenosylmethionine, S‐adenosylhomocysteine and DNA methylation

 

作者: Dorte Munksgaard,   Ole Mattsson,   Finn Thyge Okkels,  

 

期刊: Physiologia Plantarum  (WILEY Available online 1995)
卷期: Volume 93, issue 1  

页码: 5-10

 

ISSN:0031-9317

 

年代: 1995

 

DOI:10.1034/j.1399-3054.1995.930102.x

 

出版商: Munksgaard International Publishers

 

关键词: Carrot cell suspension;Daucus carota;DNA methylation;methylation index;S‐adenosylhomocysteine;S‐adenosylmethionine;somatic embryogenesis

 

数据来源: WILEY

 

摘要:

Almost homogeneous populations representing different developmental stages of somatic embryos (globular, torpedo‐shaped, plantlets) and vacuolated cells were obtained from a cell suspension culture of carrot. The concentrations of S‐adenosylmethionine (SAM), S‐adenosylhomocysteine (SAH) and methylated DNA were determined in embryos at different developmental stages and were found to increase during somatic embryogenesis. The highest increase during embryogenesis was a 5‐fold increase in the level of SAM. A considerable increase in the methylation index (SAM/SAH ratio) was also found. We propose that the levels of SAM and SAH may be involved in the control of somatic embryogenesis by affecting the level of DNA methylation, which in turn might cause differential changes in gene activation. An increase in the level of SAM may be a prerequisite for progression of embryogenesis and the development of complete

 

点击下载:  PDF (471KB)



返 回