A simplified method for the preparation of wga‐sepharose 4B and its use in the purification of MN blood group antigens
作者:
Zhang Shimin,
Li Jianxin,
Wei Yaomei,
Yang Zhiming,
期刊:
Biomedical Chromatography
(WILEY Available online 1989)
卷期:
Volume 3,
issue 1
页码: 10-13
ISSN:0269-3879
年代: 1989
DOI:10.1002/bmc.1130030104
出版商: John Wiley&Sons, Ltd.
数据来源: WILEY
摘要:
AbstractA simplified method for the preparation of wheat germ agglutinin(WGA)‐Sepharose 4B by coupling highly purified WGA, prepared by improved affinity chromatography, with BrCN activated Sepharose 4B in a solution of high carbonate buffer is described. The amount of WGA linked to Sepharose 4B was 82.40% (3.07 mg WGA per ml Sepharose 4B). MN blood group antigens of human erythrocyte membranes purified with WGA‐Sepharose 4B affinity chromatography showed a single band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE). The yield of the antigens from 400 mL fresh blood was 32–40 mg. The WGA‐Sepharose 4B column could be used several times without loss of a
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