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Avoiding Gas Bubble Formation During Freeze Substitution

 

作者: FisherDonald B.,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1991）
卷期: Volume 66, issue 5  

页码: 257-258

 

ISSN:1052-0295

 

年代: 1991

 

DOI:10.3109/10520299109109982

 

出版商: Taylor&Francis

 

关键词: freeze substitution;bubbles

 

数据来源: Taylor

 

摘要:

Gas bubbles frequently are formed during freeze substitution, especially when tissues are warmed to room temperature. The problem arises largely from the extreme solubility of CO2in the freeze substitution solvent. Gas bubbles may be minimized by briefly transferring the tissue to freshly chilled solvent before warming to room temperature.

 

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