Two 9-month continuous-flow bioassays and several intermediate length continuous-flow tests were conducted to determine safe levels of Aroclor 1242, 1248, and 1254 for the fathead minnow (Pimephales promelas) and Aroclor 1248 for the flagfish, Jordanella floridae. Calculated 96-hr LC50 values for newly hatched fathead minnows were 7.7 μg/liter for Aroclor 1254 and 15 μg/liter for 1242. Three-month-old fatheads had a 96-hr LC50 of 300 μg/liter for 1242. Reproduction occurred at and below 1.8 μg/liter 1254 and at and below 5.4 μg/liter 1242. Newly hatched young were the most sensitive life stage. Growth of young fatheads was also affected above 2.2 μg/liter 1248, and none survived above 5.1 μg/liter after 30 days. Young flagfish did not survive at 1248 concentrations above 5.1 μg/liter and did not grow well above 2.2 μg/liter.