AbstractSingle motor units of the cat's lateral rectus muscle were activated with short intracellular current pulses delivered through an intracellular micropipette penetrating a single motoneuron, and the mechanical responses in the muscle were recorded. Lateral rectus unit responses fell within the same range as those obtained earlier with extracellular stimulation of inferior oblique units, confirming that in the latter case single and not multiple motor units had been studied. It was also found that both fast and slow contracting muscle units were connected to fast conducting, large size motoneurons. The organization of motor unit recruitment in eye movements would thus seem different from the recruitment pattern in limb muscles.Intracellular stimulation of abducens nucleus “interneurons” did not initiate contractions at the ipsilateral lateral rectus muscle. These “interneurons”, therefore, did not appear to have excitatory inputs to the abducens motoneurons, but may be involved in relaying information to the other extraocular muscle nuclei or to other brain