Three‐dimensional analysis demonstrates the presence of exocrine cytoplasmic vela between endocrine cells and basal lamina in the stomach of mammals
作者:
Gilbert Pradal,
Philippe Piloquet,
Ngoc Huong Vo,
Gérard Lefranc,
期刊:
Biology of the Cell
(WILEY Available online 1991)
卷期:
Volume 71,
issue 3
页码: 297-305
ISSN:0248-4900
年代: 1991
DOI:10.1016/0248-4900(91)90274-Q
出版商: Blackwell Publishing Ltd
关键词: Three‐dimensional analysis;stomach;endocrine cells;exocrine cells;ultrastructure basal lamina
数据来源: WILEY
摘要:
Summary—Three‐dimensional analysis demonstrated the presence of cytoplasmic vela extending from exocrine cells into the space between endocrine cells and basal lamina in the gastrointestinal epithelium of the rabbit; these structures were also observed in various other mammals. The following techniques were used to determine the morphologic characteristics of these vela and to study their significance: preparation of semiserial thin sections, three‐dimensional reconstruction in plexiglass and lanthanum staining of pericellular spaces. It was found that these fine vela, devoid of major differentiated cell‐constituents, sometimes form a pseudocircular crown at the base of endocrine cells. If the zone of basal apposition of the plasma membrane is referred to as ZBA and the zones of lateral apposition as ZLA, the presence of this velum makes it possible to distinguish a zone of immediate apposition without interposition (ZIA) and a mediate zone of apposition with interposition (ZMA) within the ZBA. Exocrine cell processes can also penetrate within endocrine cells in invaginations, and the depth of these invaginations can be demonstrated by lanthanum staining. Adjacent to the membrane zones defined above, other cytoplasmic microdomains‐M(ZLA) and M(ZBA), as well as M(ZIA) and M(ZMA) of different morphofunctional significance may also be
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