Immunologic Identification of Lymphocyte Subsets in Experimental Murine Myocarditis With Encephalomyocarditis VirusDifferent Kinetics of Lymphocyte Subsets Between the Heart and the Peripheral Blood, and Significance of Thy 1.2+(Pan T) and Lyt 1+, 23+(Immature T) Subsets in the Development of Myocarditis
作者:
Chiharu Kishimoto,
Kagemasa Kuribayashi,
Kanzo Fukuma,
Tohru Masuda,
Nobuyoshi Tomioka,
Walter Abelmann,
Chuichi Kawai,
期刊:
Circulation Research
(OVID Available online 1987)
卷期:
Volume 61,
issue 5
页码: 715-725
ISSN:0009-7330
年代: 1987
出版商: OVID
关键词: myocarditis;encephalomyocarditis virus;monoclonal antibodies;lymphocyte subsets;flow cytometry;immunoperoxidase staining
数据来源: OVID
摘要:
To clarify the immune mechanism in myocarditis, immunofluorescence techniques with laser flow cytometry were used to examine serial changes in lymphocyte subsets in the heart, spleen, and peripheral blood of DBA/2 and BALB/c mice inoculated with encephalomyocarditis virus (Experiment I). B cells were identified by staining with fluorescein isothiocyanate-labelled rabbit anti-mouse immunoglobulin. T-cell subsets were identified with rat anti-Thy 1.2, and nonpolymorphic Lyt 1 and Lyt 2 monoclonal antibodies plus fluorescein isothiocyanate-labelled anti-mouse immunoglobulin. On days 7 and 14 postinfection, the percentage of Thy 1.2+(pan T) cells in both strains had decreased in the peripheral blood; B cells showed no significant changes throughout the entire period. On the other hand, Thy 1.2+(pan T) and Lyt 1+, 23+(precursor and immature) T cells appeared to occupy the major portion of the myocardium on days 7 and 14 when congestive heart failure developed. To confirm this, serial immunohistologic studies (immunoperoxidase staining) of the hearts of DBA/2 and BALB/c mice with encephalomyocarditis virus-induced myocarditis were performed (Experiment II). In Experiment II, most of the stained cells in the hearts of both strains were Thy 1.2 positive and Lyt 1 and Lyt 2 positive on days 7 and 14. Thus, Experiments I and II demonstrated that lymphocytes at the site of inflammation in acute viral myocarditis carried antigenic markers that differed from those of peripheral lymphocytes and suggested that Thy 1.2+(pan T) cells, especially the Lyt 1+, 23+subset (immature T cells and T-cell subset precursors) were involved in the development of myocarditis in these animals. (Circulation Research 1987;61:715-725)
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