Isolation and Characterization of DNA Sequences from Flow-Sorted Human Chromosome 22 Libraries
作者:
Reinhold Metzdorf,
Ruth Herzog,
Elisabeth Götten,
Nikolaus Blin,
期刊:
Human Heredity
(Karger Available online 1990)
卷期:
Volume 40,
issue 3
页码: 145-152
ISSN:0001-5652
年代: 1990
DOI:10.1159/000153922
出版商: S. Karger AG
关键词: Chromosome 22;DNA polymorphism;Mapping;DNA libraries
数据来源: Karger
摘要:
Two flow-sorted chromosome 22 libraries were used to isolate DNA sequences specific for chromosome 22. 45-phage DNAs were probed against human genomic DNA. 12 of them showed unique or low-copy character. Using digested DNA from rodent-human hybrid cell lines, 3 of the 12 recombinants were assigned unique to chromosome 22 and regionally mapped. 1 clone mapped to 22pter-q11, 1 clone to 22q12-qter and 1 clone, for which in situ hybridization was performed, to 22q13.1. 2 low-copy probes, 1 of them displaying polymorphisms in Mspl and TaqI digests of individual DNAs, must have similar sequences on 22 and additional chromosomes. Furthermore, a highly repetitive DNA representing a compound locus of some hundred kilobases on chromosome 22 was isolated. These 6 probes may provide useful tools for studying the structure and function of this small chromosome involved in a relatively high number of inherited and acquired diseases.
点击下载:
PDF
(1032KB)
返 回