Cellular distribution of inorganic mercury and its relation to cytotoxicity in bovine kidney cell cultures
作者:
W. M. Bracken,
R. P. Sharma,
D. R. Bourcier,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1984)
卷期:
Volume 13,
issue 4-6
页码: 865-877
ISSN:0098-4108
年代: 1984
DOI:10.1080/15287398409530547
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A bovine kidney cell culture system was used to assess what relationship mercuric chloride (HgCl2) uptake and subcellular distribution had to cytotoxicity. Twenty‐four‐hour incubations with 0.05–50 μMHgCl2elicited a concentration‐related cytotoxicity. Cellular accumulation of203Hg was also concentration‐related, with 1.0 nmol/106cells at the IC50. Measurement of Hg uptake over the 24‐h exposure period revealed a multiphasic process. Peak accumulation was attained by 1 h and was followed by extrusion and plateauing of intracellular Hg levels. Least‐squares regression analysis of the cytotoxicity and cellular uptake data indicated a potential relationship between the Hg uptake and cytotoxicity (r = 0.91). However, the subcellular distribution of Hg was not concentration‐related. Mitochondria and soluble protein fractions accounted for greater than 65% of the cell‐associated Hg at all concentrations. The remaining Hg was distributed between the microsomal (6–10%) and nuclear and cell debris (11–22%) fractions at all concentrations tested. Less than 20% of the total cell‐associated Hg was bound with metallothionein‐like protein.
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