Inhibition of Receptor‐Dependent and Receptor‐Independent Generation of the Respiratory Burst in Human Neutrophils and Monocytes by Human Serum IgA
作者:
HERMANN WOLF,
ERICH VOGEL,
MICHAEL FISCHER,
HENRI RENGS,
HANS-PETER SCHWARZ,
MARTHA EIBL,
期刊:
Pediatric Research
(OVID Available online 1994)
卷期:
Volume 36,
issue 2
页码: 235-243
ISSN:0031-3998
年代: 1994
出版商: OVID
数据来源: OVID
摘要:
An important feature of the role of IgA in protection against infection and disease at the level of the mucosal surfaces might be the elimination of pathogens without induction of a strong inflammatory reaction. In the present study we addressed the question whether IgA has a regulatory effect on the generation of reactive oxygen intermediates in human neutrophils and monocytes (i.e. the respiratory burst). Cells were stimulated with heat-inactivatedHaemophilus influenzaetype b or phorbol myristate acetate, stimuli known to use different recognition structures or signal transduction pathways. Concentrations of IgA as low as 10 mg/L significantly inhibited the receptor-dependentHaemophilus influenzae-induced respiratory burst in granulocytes, as assessed by measuring luminol-enhanced chemiluminescence. Furthermore, IgA had a dose-dependent inhibitory effect on the receptor-independent induction of the respiratory burst, as examined by flow cytometry in monocytes and granulocytes activated with phorbol myristate acetate. Our results therefore indicate that inhibition of receptor-ligand interaction is not a sufficient explanation for the IgA-mediated modulation of the respiratory burst in human phagocytic cells. In addition, IgA might directly regulate the activation of the respiratory burst at the level or downstream of protein kinase C activation. By modulating the release of mediators of inflammation such as reactive oxygen intermediates, the inflammatory response could be down-regulated at the level of the mucosal surfaces, thereby preventing the development of sequelae of an exaggerated inflammatory response potentially leading to local or systemic pathology.
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