Cholinergic Stimulation of Inositol Phosphate Production in Cultured Anterior Pituitary Cells
作者:
P. Luigi Canonico,
David Jarvis,
Maria Angela Sortino,
Umberto Scapagnini,
Robert M. MacLeod,
期刊:
Neuroendocrinology
(Karger Available online 1987)
卷期:
Volume 46,
issue 4
页码: 306-311
ISSN:0028-3835
年代: 1987
DOI:10.1159/000124837
出版商: S. Karger AG
关键词: Acetylcholine;Carbachol;Pituitary;Polyphosphoinositides
数据来源: Karger
摘要:
The effects of acetylcholine and of the muscarinic receptor agonist carbachol on inositol phosphate production were studied in cultured rat anterior pituitary cells. In the presence of the cholinesterase inhibitor physostigmine, acetylcholine significantly (p < 0.05–p < 0.01) stimulated inositol phosphate formation in a concentration-related fashion; carbachol, but not oxotremorine, produced similar effects. The increase in the amount of inositol phosphates (primarily inositol trisphosphate and inositol bisphosphate) was very rapid, an effect potently antagonized by the muscarinic receptor antagonist atropine. This agent significantly attenuated the stimulatory effect of carbachol on growth hormone (GH) release. These results indicate that the effects exerted by acetylcholine on anterior pituitary function (i.e. GH release) may be mediated, at least in part, by receptor-activated polyphosphoinositide hydrolysis. In addition, acetylcholine and car-bachol’s relation with other intracellular pathways and with hormone release is discus
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