Mutation and expression of thep53gene in human malignant melanoma
作者:
A P Albino,
M J Vidal,
N S McNutt,
C R Shea,
V G Prieto,
D M Nanus,
J M Palmer,
N K Hayward,
期刊:
Melanoma Research
(OVID Available online 1994)
卷期:
Volume 4,
issue 1
页码: 35-45
ISSN:0960-8931
年代: 1994
出版商: OVID
关键词: Malignant melanoma;melanocyte;mutation;p53
数据来源: OVID
摘要:
Derangement of thep53tumor suppressor gene has been implicated in the aetiology of a wide range of human neoplasias. We have previously determined that overexpression and mutation of thep53gene in cultured metastatic melanomas is low (11%). However, two recent immunohistochemical studies have reported that >85% of malignant melanoma specimens overexpress mutated p53 protein. In an effort to resolve this contradiction in the published literature, we have re-evaluated a range of cultured and non-cultured melanocytic lesions for the occurrence of point mutations in thep53gene using DNA- and RNA-dependent single strand conformation polymorphism (RNA-SSCP) and direct DNA sequencing of polymerase chain reaction (PCR) amplified DNA, and overexpression of the p53 protein using immunohistochemistry. We found point mutations in 25% (9 of 36) of cultured melanomas and 0% in 34 fresh melanoma biopsies; however, increased p53 expression was found in 42% of paraffin-embedded melanoma specimens and 7% of benign lesions. The low frequency ofp53point mutations and high frequency of p53 expression suggests that derangement of thep53gene by point mutations is not a common perturbation in the majority of melanoma cells, and that overexpression of p53 in this tumour type is due to a mechanism other than point mutation.
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