Monoclonal antibodies to rabbit corneal keratan sulfate proteoglycan
作者:
FunderburghJames L.,
StenzelPaule R.,
ChandlerJohn W.,
期刊:
Current Eye Research
(Taylor Available online 1982)
卷期:
Volume 2,
issue 11
页码: 769-776
ISSN:0271-3683
年代: 1982
DOI:10.3109/02713688209020010
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Six mouse hybrid cell lines were isolated which secrete antibodies to rabbit corneal proteoglycan. All six antibodies interacted with the same fraction of the proteoglycan, precipitating approximately 50% of proteoglycan labelled in the protein moiety. A radioimmunoassay using these antibodies measured concentrations as low as 1 g/m1 unlabelled rabbit corneal proteoglycan. Human corneal proteoglycanj corneal keratan sulfate, and an oligosaccharide fraction from corneal digests all interacted with the antibodies at concentrations similar to whole rabbit proteoglycan. Proteoglycans from cultured rabbit stromal fibroblasts and from sclera were 20 to 50-fold less effective in competition for antibody. Endo-β-galactosidase treatment of proteoglycan reduced antibody binding, but protease or chondroitinase treatments did not. Labelled proteoglycan separated by antibody affinity chromatography contained only keratan sulfate, whereas proteoglycan not bound to affinity columns contained only chondroitin sulfate. The antibodies appear to recognise a carbohydrate structure found only on corneal keratan sulfate proteoglycan. This structure can serve as a basis for separation of corneal proteoglycan types using antibody affinity chromatography.
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